Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [16]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-395 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-395, RRID:AB_10001489
- Product name
- Rabbit Polyclonal MDC1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Coordination of the recruitment of the FANCD2 and PALB2 Fanconi anemia proteins by an ubiquitin signaling network.
Femtosecond near-infrared laser microirradiation reveals a crucial role for PARP signaling on factor assemblies at DNA damage sites.
RNF8 E3 Ubiquitin Ligase Stimulates Ubc13 E2 Conjugating Activity That Is Essential for DNA Double Strand Break Signaling and BRCA1 Tumor Suppressor Recruitment.
The UBC Domain Is Required for BRUCE to Promote BRIT1/MCPH1 Function in DSB Signaling and Repair Post Formation of BRUCE-USP8-BRIT1 Complex.
Listeria monocytogenes dampens the DNA damage response.
The tumor suppressor PML specifically accumulates at RPA/Rad51-containing DNA damage repair foci but is nonessential for DNA damage-induced fibroblast senescence.
DNA damage to a single chromosome end delays anaphase onset.
The in vivo dynamic interplay of MDC1 and 53BP1 at DNA damage-induced nuclear foci.
CK2 inhibitor CX-4945 suppresses DNA repair response triggered by DNA-targeted anticancer drugs and augments efficacy: mechanistic rationale for drug combination therapy.
Survivin inhibition and DNA double-strand break repair: a molecular mechanism to overcome radioresistance in glioblastoma.
Mild hyperthermia inhibits homologous recombination, induces BRCA2 degradation, and sensitizes cancer cells to poly (ADP-ribose) polymerase-1 inhibition.
Wild-type p53-induced phosphatase 1 dephosphorylates histone variant gamma-H2AX and suppresses DNA double strand break repair.
Competition effect in DNA damage response.
Disassembly of MDC1 foci is controlled by ubiquitin-proteasome-dependent degradation.
ATM-dependent phosphorylation of ATF2 is required for the DNA damage response.
The BRCA1 RING and BRCT domains cooperate in targeting BRCA1 to ionizing radiation-induced nuclear foci.
Bick G, Zhang F, Meetei AR, Andreassen PR
Chromosoma 2017 Jun;126(3):417-430
Chromosoma 2017 Jun;126(3):417-430
Femtosecond near-infrared laser microirradiation reveals a crucial role for PARP signaling on factor assemblies at DNA damage sites.
Saquilabon Cruz GM, Kong X, Silva BA, Khatibzadeh N, Thai R, Berns MW, Yokomori K
Nucleic acids research 2016 Feb 18;44(3):e27
Nucleic acids research 2016 Feb 18;44(3):e27
RNF8 E3 Ubiquitin Ligase Stimulates Ubc13 E2 Conjugating Activity That Is Essential for DNA Double Strand Break Signaling and BRCA1 Tumor Suppressor Recruitment.
Hodge CD, Ismail IH, Edwards RA, Hura GL, Xiao AT, Tainer JA, Hendzel MJ, Glover JN
The Journal of biological chemistry 2016 Apr 29;291(18):9396-410
The Journal of biological chemistry 2016 Apr 29;291(18):9396-410
The UBC Domain Is Required for BRUCE to Promote BRIT1/MCPH1 Function in DSB Signaling and Repair Post Formation of BRUCE-USP8-BRIT1 Complex.
Ge C, Che L, Du C
PloS one 2015;10(12):e0144957
PloS one 2015;10(12):e0144957
Listeria monocytogenes dampens the DNA damage response.
Samba-Louaka A, Pereira JM, Nahori MA, Villiers V, Deriano L, Hamon MA, Cossart P
PLoS pathogens 2014 Oct;10(10):e1004470
PLoS pathogens 2014 Oct;10(10):e1004470
The tumor suppressor PML specifically accumulates at RPA/Rad51-containing DNA damage repair foci but is nonessential for DNA damage-induced fibroblast senescence.
Münch S, Weidtkamp-Peters S, Klement K, Grigaravicius P, Monajembashi S, Salomoni P, Pandolfi PP, Weißhart K, Hemmerich P
Molecular and cellular biology 2014 May;34(10):1733-46
Molecular and cellular biology 2014 May;34(10):1733-46
DNA damage to a single chromosome end delays anaphase onset.
Silva BA, Stambaugh JR, Yokomori K, Shah JV, Berns MW
The Journal of biological chemistry 2014 Aug 15;289(33):22771-22784
The Journal of biological chemistry 2014 Aug 15;289(33):22771-22784
The in vivo dynamic interplay of MDC1 and 53BP1 at DNA damage-induced nuclear foci.
Mok MT, Henderson BR
The international journal of biochemistry & cell biology 2012 Sep;44(9):1398-409
The international journal of biochemistry & cell biology 2012 Sep;44(9):1398-409
CK2 inhibitor CX-4945 suppresses DNA repair response triggered by DNA-targeted anticancer drugs and augments efficacy: mechanistic rationale for drug combination therapy.
Siddiqui-Jain A, Bliesath J, Macalino D, Omori M, Huser N, Streiner N, Ho CB, Anderes K, Proffitt C, O'Brien SE, Lim JK, Von Hoff DD, Ryckman DM, Rice WG, Drygin D
Molecular cancer therapeutics 2012 Apr;11(4):994-1005
Molecular cancer therapeutics 2012 Apr;11(4):994-1005
Survivin inhibition and DNA double-strand break repair: a molecular mechanism to overcome radioresistance in glioblastoma.
Reichert S, Rödel C, Mirsch J, Harter PN, Tomicic MT, Mittelbronn M, Kaina B, Rödel F
Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology 2011 Oct;101(1):51-8
Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology 2011 Oct;101(1):51-8
Mild hyperthermia inhibits homologous recombination, induces BRCA2 degradation, and sensitizes cancer cells to poly (ADP-ribose) polymerase-1 inhibition.
Krawczyk PM, Eppink B, Essers J, Stap J, Rodermond H, Odijk H, Zelensky A, van Bree C, Stalpers LJ, Buist MR, Soullié T, Rens J, Verhagen HJ, O'Connor MJ, Franken NA, Ten Hagen TL, Kanaar R, Aten JA
Proceedings of the National Academy of Sciences of the United States of America 2011 Jun 14;108(24):9851-6
Proceedings of the National Academy of Sciences of the United States of America 2011 Jun 14;108(24):9851-6
Wild-type p53-induced phosphatase 1 dephosphorylates histone variant gamma-H2AX and suppresses DNA double strand break repair.
Moon SH, Lin L, Zhang X, Nguyen TA, Darlington Y, Waldman AS, Lu X, Donehower LA
The Journal of biological chemistry 2010 Apr 23;285(17):12935-47
The Journal of biological chemistry 2010 Apr 23;285(17):12935-47
Competition effect in DNA damage response.
Greubel C, Hable V, Drexler GA, Hauptner A, Dietzel S, Strickfaden H, Baur I, Krücken R, Cremer T, Dollinger G, Friedl AA
Radiation and environmental biophysics 2008 Nov;47(4):423-9
Radiation and environmental biophysics 2008 Nov;47(4):423-9
Disassembly of MDC1 foci is controlled by ubiquitin-proteasome-dependent degradation.
Shi W, Ma Z, Willers H, Akhtar K, Scott SP, Zhang J, Powell S, Zhang J
The Journal of biological chemistry 2008 Nov 14;283(46):31608-16
The Journal of biological chemistry 2008 Nov 14;283(46):31608-16
ATM-dependent phosphorylation of ATF2 is required for the DNA damage response.
Bhoumik A, Takahashi S, Breitweiser W, Shiloh Y, Jones N, Ronai Z
Molecular cell 2005 May 27;18(5):577-87
Molecular cell 2005 May 27;18(5):577-87
The BRCA1 RING and BRCT domains cooperate in targeting BRCA1 to ionizing radiation-induced nuclear foci.
Au WW, Henderson BR
The Journal of biological chemistry 2005 Feb 25;280(8):6993-7001
The Journal of biological chemistry 2005 Feb 25;280(8):6993-7001
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MDC1 Antibody [NB100-395] - Samples: Whole cell lysate (15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MDC1 antibody NB100-395 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MDC1 Antibody [NB100-395] - Human lung cancer cell lines (UT) Untreated and (Cis) 10uM Cisplatin 16h. Image from verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: MDC1 Antibody [NB100-395] - Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MDC1 antibody NB100-395 used for IP at 3 ug per reaction. MDC1 was also immunoprecipitated by rabbit anti-MDC1 antibody NB100-396. For blotting immunoprecipitated MDC1, NB100-395 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
