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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [22]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB190-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CXCR5 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Stains human CXCR5 transfectants but not the parental cell lines in flow cytometry. Does not cross-react with human CXCR2, CXCR3, or CXCR4 transfectants.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P32302- Isotype
- IgG
- Antibody clone number
- 51505
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Diminished CXCR5 expression in peripheral blood of patients with Sjögren's syndrome may relate to both genotype and salivary gland homing.
Role of CXCL13 and CCL20 in the recruitment of B cells to inflammatory foci in chronic arthritis.
p53-dependent expression of CXCR5 chemokine receptor in MCF-7 breast cancer cells.
The MEC1 and MEC2 lines represent two CLL subclones in different stages of progression towards prolymphocytic leukemia.
CXCR7 influences the migration of B cells during maturation.
Capture-stabilize approach for membrane protein SPR assays.
IL-15 positively regulates IL-21 production in celiac disease mucosa.
Blood CD4+CD45RO+CXCR5+ T cells are decreased but partially functional in signal transducer and activator of transcription 3 deficiency.
Mouse CCL8, a CCR8 agonist, promotes atopic dermatitis by recruiting IL-5+ T(H)2 cells.
IL8 and CXCL13 are potent chemokines for the recruitment of human neural precursor cells across brain endothelial cells.
Serum CXCL13 positively correlates with prostatic disease, prostate-specific antigen and mediates prostate cancer cell invasion, integrin clustering and cell adhesion.
Chemokine CXCL13 is overexpressed in the tumour tissue and in the peripheral blood of breast cancer patients.
Altered expression of the receptor-ligand pair CXCR5/CXCL13 in B cells during chronic HIV-1 infection.
Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells.
The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities.
Hyaluronan-based polymer scaffold modulates the expression of inflammatory and degradative factors in mesenchymal stem cells: Involvement of Cd44 and Cd54.
Chemokine receptor expression in rat adjuvant-induced arthritis.
Developing neonatal rabbit appendix, a primary lymphoid organ, is seeded by immature blood-borne B cells: evidence for roles for CD62L/PNAd, CCR7/CCL21, alpha4beta1 and LFA-1.
Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses.
Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL.
Expression of functional CCR and CXCR chemokine receptors in podocytes.
Unique subpopulations of CD56+ NK and NK-T peripheral blood lymphocytes identified by chemokine receptor expression repertoire.
Aqrawi LA, Ivanchenko M, Björk A, Ramírez Sepúlveda JI, Imgenberg-Kreuz J, Kvarnström M, Haselmayer P, Jensen JL, Nordmark G, Chemin K, Skarstein K, Wahren-Herlenius M
Clinical and experimental immunology 2018 Jun;192(3):259-270
Clinical and experimental immunology 2018 Jun;192(3):259-270
Role of CXCL13 and CCL20 in the recruitment of B cells to inflammatory foci in chronic arthritis.
Armas-González E, Domínguez-Luis MJ, Díaz-Martín A, Arce-Franco M, Castro-Hernández J, Danelon G, Hernández-Hernández V, Bustabad-Reyes S, Cantabrana A, Uguccioni M, Díaz-González F
Arthritis research & therapy 2018 Jun 7;20(1):114
Arthritis research & therapy 2018 Jun 7;20(1):114
p53-dependent expression of CXCR5 chemokine receptor in MCF-7 breast cancer cells.
Mitkin NA, Hook CD, Schwartz AM, Biswas S, Kochetkov DV, Muratova AM, Afanasyeva MA, Kravchenko JE, Bhattacharyya A, Kuprash DV
Scientific reports 2015 Mar 19;5:9330
Scientific reports 2015 Mar 19;5:9330
The MEC1 and MEC2 lines represent two CLL subclones in different stages of progression towards prolymphocytic leukemia.
Rasul E, Salamon D, Nagy N, Leveau B, Banati F, Szenthe K, Koroknai A, Minarovits J, Klein G, Klein E
PloS one 2014;9(8):e106008
PloS one 2014;9(8):e106008
CXCR7 influences the migration of B cells during maturation.
Humpert ML, Pinto D, Jarrossay D, Thelen M
European journal of immunology 2014 Mar;44(3):694-705
European journal of immunology 2014 Mar;44(3):694-705
Capture-stabilize approach for membrane protein SPR assays.
Chu R, Reczek D, Brondyk W
Scientific reports 2014 Dec 8;4:7360
Scientific reports 2014 Dec 8;4:7360
IL-15 positively regulates IL-21 production in celiac disease mucosa.
Sarra M, Cupi ML, Monteleone I, Franzè E, Ronchetti G, Di Sabatino A, Gentileschi P, Franceschilli L, Sileri P, Sica G, Del Vecchio Blanco G, Cretella M, Paoluzi OA, Corazza GR, Pallone F, Monteleone G
Mucosal immunology 2013 Mar;6(2):244-55
Mucosal immunology 2013 Mar;6(2):244-55
Blood CD4+CD45RO+CXCR5+ T cells are decreased but partially functional in signal transducer and activator of transcription 3 deficiency.
Mazerolles F, Picard C, Kracker S, Fischer A, Durandy A
The Journal of allergy and clinical immunology 2013 Apr;131(4):1146-56, 1156.e1-5
The Journal of allergy and clinical immunology 2013 Apr;131(4):1146-56, 1156.e1-5
Mouse CCL8, a CCR8 agonist, promotes atopic dermatitis by recruiting IL-5+ T(H)2 cells.
Islam SA, Chang DS, Colvin RA, Byrne MH, McCully ML, Moser B, Lira SA, Charo IF, Luster AD
Nature immunology 2011 Feb;12(2):167-77
Nature immunology 2011 Feb;12(2):167-77
IL8 and CXCL13 are potent chemokines for the recruitment of human neural precursor cells across brain endothelial cells.
Weiss N, Deboux C, Chaverot N, Miller F, Baron-Van Evercooren A, Couraud PO, Cazaubon S
Journal of neuroimmunology 2010 Jun;223(1-2):131-4
Journal of neuroimmunology 2010 Jun;223(1-2):131-4
Serum CXCL13 positively correlates with prostatic disease, prostate-specific antigen and mediates prostate cancer cell invasion, integrin clustering and cell adhesion.
Singh S, Singh R, Sharma PK, Singh UP, Rai SN, Chung LW, Cooper CR, Novakovic KR, Grizzle WE, Lillard JW Jr
Cancer letters 2009 Sep 28;283(1):29-35
Cancer letters 2009 Sep 28;283(1):29-35
Chemokine CXCL13 is overexpressed in the tumour tissue and in the peripheral blood of breast cancer patients.
Panse J, Friedrichs K, Marx A, Hildebrandt Y, Luetkens T, Barrels K, Horn C, Stahl T, Cao Y, Milde-Langosch K, Niendorf A, Kröger N, Wenzel S, Leuwer R, Bokemeyer C, Hegewisch-Becker S, Atanackovic D
British journal of cancer 2008 Sep 16;99(6):930-8
British journal of cancer 2008 Sep 16;99(6):930-8
Altered expression of the receptor-ligand pair CXCR5/CXCL13 in B cells during chronic HIV-1 infection.
Cagigi A, Mowafi F, Phuong Dang LV, Tenner-Racz K, Atlas A, Grutzmeier S, Racz P, Chiodi F, Nilsson A
Blood 2008 Dec 1;112(12):4401-10
Blood 2008 Dec 1;112(12):4401-10
Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells.
Kang SG, Lim HW, Andrisani OM, Broxmeyer HE, Kim CH
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):3724-33
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):3724-33
The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities.
Ponte AL, Marais E, Gallay N, Langonné A, Delorme B, Hérault O, Charbord P, Domenech J
Stem cells (Dayton, Ohio) 2007 Jul;25(7):1737-45
Stem cells (Dayton, Ohio) 2007 Jul;25(7):1737-45
Hyaluronan-based polymer scaffold modulates the expression of inflammatory and degradative factors in mesenchymal stem cells: Involvement of Cd44 and Cd54.
Lisignoli G, Cristino S, Piacentini A, Cavallo C, Caplan AI, Facchini A
Journal of cellular physiology 2006 May;207(2):364-73
Journal of cellular physiology 2006 May;207(2):364-73
Chemokine receptor expression in rat adjuvant-induced arthritis.
Haas CS, Martinez RJ, Attia N, Haines GK 3rd, Campbell PL, Koch AE
Arthritis and rheumatism 2005 Dec;52(12):3718-30
Arthritis and rheumatism 2005 Dec;52(12):3718-30
Developing neonatal rabbit appendix, a primary lymphoid organ, is seeded by immature blood-borne B cells: evidence for roles for CD62L/PNAd, CCR7/CCL21, alpha4beta1 and LFA-1.
Sinha RK, Mage RG
Developmental and comparative immunology 2004 Jun;28(7-8):829-41
Developmental and comparative immunology 2004 Jun;28(7-8):829-41
Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses.
Lim HW, Hillsamer P, Kim CH
The Journal of clinical investigation 2004 Dec;114(11):1640-9
The Journal of clinical investigation 2004 Dec;114(11):1640-9
Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL.
Annels NE, Willemze AJ, van der Velden VH, Faaij CM, van Wering E, Sie-Go DM, Egeler RM, van Tol MJ, Révész T
Blood 2004 Apr 1;103(7):2806-8
Blood 2004 Apr 1;103(7):2806-8
Expression of functional CCR and CXCR chemokine receptors in podocytes.
Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstädt H
Journal of immunology (Baltimore, Md. : 1950) 2002 Jun 15;168(12):6244-52
Journal of immunology (Baltimore, Md. : 1950) 2002 Jun 15;168(12):6244-52
Unique subpopulations of CD56+ NK and NK-T peripheral blood lymphocytes identified by chemokine receptor expression repertoire.
Campbell JJ, Qin S, Unutmaz D, Soler D, Murphy KE, Hodge MR, Wu L, Butcher EC
Journal of immunology (Baltimore, Md. : 1950) 2001 Jun 1;166(11):6477-82
Journal of immunology (Baltimore, Md. : 1950) 2001 Jun 1;166(11):6477-82
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CXCR5 in Human PBMCs. CXCR5 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CXCR5 Monoclonal Antibody (Catalog # MAB190) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 637-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL008) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CXCR5 in Human Kidney. CXCR5 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human CXCR5 Monoclonal Antibody (Catalog # MAB190) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CXCR5 in CD19+ Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human CXCR5 Monoclonal Antibody (Catalog # MAB190) or (B) Mouse IgG2B control antibody (Catalog # MAB0041) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CXCL13/BLC/BCA-1 and Neutralization by Human CXCR5 Antibody. Recombinant Human CXCL13/BLC/BCA-1 (Catalog # 801-CX) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL13/BLC/BCA-1 (0.05 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR5 Monoclonal Antibody (Catalog # MAB190). The ND50 is typically 0.25-1.5 µg/mL.
