H00000643-D01P

antibody from Abnova Corporation

Targeting: CXCR5 BLR1, CD185, MDR15

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000643-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000643-D01P, RRID:AB_1673310

Product name

CXCR5 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human CXCR5 protein.

Antigen sequence

MNYPLTLEMDLENLEDLFWELDRLDNYNDTSLVEN
HLCPATEGPLMASFKAVFVPVAYSLIFLLGVIGNV
LVLVILERHRQTRSSTETFLFHLAVADLLLVFILP
FAVAEGSVGWVLGTFLCKTVIALHKVNFYCSSLLL
ACIAVDRYLAIVHAVHAYRHRRLLSIHITCGTIWL
VGFLLALPEILFAKVSQGHHNNSLPRCTFSQENQA
ETHAWFTSRFLYHVAGFLLPMLVMGWCYVGVVHRL
RQAQRRPQRQKAVRVAILVTSIFFLCWSPYHIVIF
LDTLARLKAVDNTCKLNGSLPVAITMCEFLGLAHC
CLNPMLYTFAGVKFRSDLSRLLTKLGCTGPASLCQ
LFPSWRRSSLSESENATSLTTF


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of CXCR5 expression in transfected 293T cell line (H00000643-T01) by CXCR5 MaxPab polyclonal antibody.Lane 1: CXCR5 transfected lysate(42.00 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between CXCR5 and GNAI2. HeLa cells were stained with anti-CXCR5 rabbit purified polyclonal 1:1200 and anti-GNAI2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)