Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX100094 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100094, RRID:AB_1952611
- Product name
- XRCC4 antibody [N3C3]
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Storage
- Keep as concentrated solution. Aliquot and store at -20?C or below. Avoid multiple freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: 293T B: H1299 C: HepG2 D: Molt-4 E: Raji 10% SDS PAGE GTX100094 diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using XRCC4(GTX100094) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- XRCC4 antibody immunoprecipitates XRCC4 protein in IP experiments. IP Sample: 1000 £gg 293T whole cell lysate/extract A. 40 £gg 293T whole cell lysate/extract B. Control with 2.5 £gg of preimmune rabbit IgG C. Immunoprecipitation of XRCC4 protein by 2.5 £gg of XRCC4 antibody (GTX100094) 10% SDS-PAGE The immunoprecipitated XRCC4 protein was detected by XRCC4 antibody (GTX100094) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 xenograft, using XRCC4(GTX100094) antibody at 1:500 dilution.
