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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-27104 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-XRCC4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- PA5-27107 targets XRCC4 in WB applications and shows reactivity with Human samples. The PA5-27107 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 109 of Human XRCC4.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.39 mg/ml
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Mitochondrial DNA repair and replication proteins revealed by targeted chemical probes.
Wisnovsky S, Jean SR, Kelley SO
Nature chemical biology 2016 Jul;12(7):567-73
Nature chemical biology 2016 Jul;12(7):567-73
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of XRCC4 in paraformaldehyde-fixed HeLa cells using a XRCC4 polyclonal antibody (Product # PA5-27104) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of XRCC4 in paraffin-embedded A549 xenograft using a XRCC4 polyclonal antibody (Product # PA5-27104) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PA5-27104 immunoprecipitates XRCC4 protein in IP experiments. IP Sample: 1000 µg 293T whole cell lysate/extract A. 40 µg 293T whole cell lysate/extract B. Control with 2.5 µg of preimmune rabbit IgG C. Immunoprecipitation of XRCC4 protein by 2.5 µg of XRCC4 antibody (Product # PA5-27104) on 10% SDS-PAGE.
