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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-378 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-378, RRID:AB_631542
- Product name
- Anti-GNB4
- Antibody type
- Polyclonal (Antigen purified)
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
Submitted references A novel alternatively spliced isoform of the mu-opioid receptor: functional antagonism.
A novel inhibitor of the NF-{kappa}B signaling pathway encoded by the parapoxvirus orf virus.
A new type of ERK1/2 autophosphorylation causes cardiac hypertrophy
G protein beta gamma subunit interaction with the dynein light-chain component Tctex-1 regulates neurite outgrowth.
Pleiotropic phenotype of a genomic knock-in of an RGS-insensitive G184S Gnai2 allele.
Involvement of both G protein alphas and beta gamma subunits in beta-adrenergic stimulation of vascular L-type Ca(2+) channels.
On the role of endogenous G-protein beta gamma subunits in N-type Ca2+ current inhibition by neurotransmitters in rat sympathetic neurones.
Gris P, Gauthier J, Cheng P, Gibson DG, Gris D, Laur O, Pierson J, Wentworth S, Nackley AG, Maixner W, Diatchenko L
Molecular pain 2010 Jun 2;6:33
Molecular pain 2010 Jun 2;6:33
A novel inhibitor of the NF-{kappa}B signaling pathway encoded by the parapoxvirus orf virus.
Diel DG, Delhon G, Luo S, Flores EF, Rock DL
Journal of virology 2010 Apr;84(8):3962-73
Journal of virology 2010 Apr;84(8):3962-73
A new type of ERK1/2 autophosphorylation causes cardiac hypertrophy
Kristina Lorenz, Joachim P Schmitt, Eva M Schmitteckert, Martin J Lohse
Nature Medicine 2008 Dec;15(1):75-83
Nature Medicine 2008 Dec;15(1):75-83
G protein beta gamma subunit interaction with the dynein light-chain component Tctex-1 regulates neurite outgrowth.
Sachdev P, Menon S, Kastner DB, Chuang JZ, Yeh TY, Conde C, Caceres A, Sung CH, Sakmar TP
The EMBO journal 2007 Jun 6;26(11):2621-32
The EMBO journal 2007 Jun 6;26(11):2621-32
Pleiotropic phenotype of a genomic knock-in of an RGS-insensitive G184S Gnai2 allele.
Huang X, Fu Y, Charbeneau RA, Saunders TL, Taylor DK, Hankenson KD, Russell MW, D'Alecy LG, Neubig RR
Molecular and cellular biology 2006 Sep;26(18):6870-9
Molecular and cellular biology 2006 Sep;26(18):6870-9
Involvement of both G protein alphas and beta gamma subunits in beta-adrenergic stimulation of vascular L-type Ca(2+) channels.
Viard P, Macrez N, Mironneau C, Mironneau J
British journal of pharmacology 2001 Feb;132(3):669-76
British journal of pharmacology 2001 Feb;132(3):669-76
On the role of endogenous G-protein beta gamma subunits in N-type Ca2+ current inhibition by neurotransmitters in rat sympathetic neurones.
Delmas P, Brown DA, Dayrell M, Abogadie FC, Caulfield MP, Buckley NJ
The Journal of physiology 1998 Jan 15;506 ( Pt 2)(Pt 2):319-29
The Journal of physiology 1998 Jan 15;506 ( Pt 2)(Pt 2):319-29
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 38
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human stomach shows strong membranous and cytoplasmic positivity in glandular cells.
- Validation comment
- Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.
