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LearnMA5-29480
antibody from Invitrogen Antibodies
Targeting: PHPT1
bA216L13.10, CGI-202, DKFZp564M173, HSPC141, PHP14
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MA5-29480 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PHPT1 Recombinant Rabbit Monoclonal Antibody (1)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
- Antibody clone number
- 1
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of Human PHPT1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with PHPT1 Recombinant Rabbit Monoclonal Antibody (1) (Product # MA5-29480, 1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human PHPT1 in human skeletal muscle with PHPT1 Recombinant Rabbit Monoclonal Antibody (1) (Product # MA5-29480, 1:5,000, formalin-fixed paraffin embedded sections).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Human PHPT1 expression on THP1 cells . The cells were treated according to manufacturer’s manual, stained with PHPT1 Recombinant Rabbit Monoclonal Antibody (1) (Product # MA5-29480), then a FITC-conjugated Secondary antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
