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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- ABIN359046 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-G Protein-Coupled Receptor Kinase 5 (GRK5) (C-Term) antibody
- Antibody type
- Polyclonal
- Antigen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human GRK5.
- Description
- Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Epitope
- C-Term
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Synucleins are a novel class of substrates for G protein-coupled receptor kinases.
Structure-function analysis of G protein-coupled receptor kinase-5. Role of the carboxyl terminus in kinase regulation.
Involvement of G protein-coupled receptor kinase 5 in homologous desensitization of the thyrotropin receptor.
Expression, purification, and characterization of the G protein-coupled receptor kinase GRK5.
Cloning and expression of GRK5: a member of the G protein-coupled receptor kinase family.
Pronin AN, Morris AJ, Surguchov A, Benovic JL
The Journal of biological chemistry 2000 Aug 25;275(34):26515-22
The Journal of biological chemistry 2000 Aug 25;275(34):26515-22
Structure-function analysis of G protein-coupled receptor kinase-5. Role of the carboxyl terminus in kinase regulation.
Pronin AN, Carman CV, Benovic JL
The Journal of biological chemistry 1998 Nov 20;273(47):31510-8
The Journal of biological chemistry 1998 Nov 20;273(47):31510-8
Involvement of G protein-coupled receptor kinase 5 in homologous desensitization of the thyrotropin receptor.
Nagayama Y, Tanaka K, Hara T, Namba H, Yamashita S, Taniyama K, Niwa M
The Journal of biological chemistry 1996 Apr 26;271(17):10143-8
The Journal of biological chemistry 1996 Apr 26;271(17):10143-8
Expression, purification, and characterization of the G protein-coupled receptor kinase GRK5.
Kunapuli P, Onorato JJ, Hosey MM, Benovic JL
The Journal of biological chemistry 1994 Jan 14;269(2):1099-105
The Journal of biological chemistry 1994 Jan 14;269(2):1099-105
Cloning and expression of GRK5: a member of the G protein-coupled receptor kinase family.
Kunapuli P, Benovic JL
Proceedings of the National Academy of Sciences of the United States of America 1993 Jun 15;90(12):5588-92
Proceedings of the National Academy of Sciences of the United States of America 1993 Jun 15;90(12):5588-92
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of anti-GRK5 Pab in HeLa cell lysate (Lane 1) and mouse brain tissue lysate (Lane 2). GRK5 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- (LEFT)Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (RIGHT)Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GRK5 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
