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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-19240 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TIA1/TIAL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, canine and mouse based on sequence homology.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references SARS-CoV-2 N Protein Antagonizes Stress Granule Assembly and IFN Production by Interacting with G3BPs to Facilitate Viral Replication.
Liu H, Bai Y, Zhang X, Gao T, Liu Y, Li E, Wang X, Cao Z, Zhu L, Dong Q, Hu Y, Wang G, Song C, Niu X, Zheng T, Wang D, Liu Z, Jin Y, Li P, Bian X, Cao C, Liu X
Journal of virology 2022 Jun 22;96(12):e0041222
Journal of virology 2022 Jun 22;96(12):e0041222
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of K562 cell lysate using Product # PA5-19240 at a concentration of 2 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TIA1 TIAL1 in paraffin embedded human skin using a TIA1 TIAL1 polyclonal antibody (Product #PA5-19240) at a concentration of 3.8 µg/mL. Steamed antigen retrieval was performed with pH 6 buffered citrate. Samples were then stained with alkaline phosphatase.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIG 5 SARS-CoV-2 NP suppresses G3BP-mediated SG formation. (A) A549 cells were treated with 500 muM sodium arsenite (SA) for 1 h to induce SG assembly, and saline was used as a control. G3BP1 (green) and G3BP2 (red) were labeled to visualize SGs by immunofluorescence staining. The nuclei were stained with DAPI (blue). (B) A549 cells expressing GFP-tagged NP SARS-CoV-2 or GFP-vector were treated with 500 muM SA for 1 h to induce SG assembly. TIA1 (red) and G3BP1 (violet) were labeled to visualize SGs by immunofluorescence staining. The nuclei were stained with DAPI (blue). (C) The average SG number per cell (>=10) in panel B was calculated using ImageJ software. All quantitative data are shown as the means +- SDs. * * , P < 0.05. (D) A549 cells transfected with or without a G3BP1/G3BP2 plasmid were infected with SARS-CoV-2 for 24 h and treated with or without 500 muM SA during the last hour. NP SARS-CoV-2 (green) and the SG marker G3BP1 (red) were detected via immunofluorescence staining. The nuclei were stained with DAPI (blue). (E) The average SG number per cell (>=10) in panel D was calculated using ImageJ software. All quantitative data are shown as the means +- SDs. * * , P < 0.05.
