Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [3]
- Immunohistochemistry [6]
- Other assay [1]
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Validation data
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- Product number
- 13194-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FXR1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MAELTVEVR GSNGAFYKGF IKDVHEDSLT VVFENNWQPE RQVPFNEVRL PPPPDIKKEI SEGDEVEVYS RANDQEPCGW WLAKVRMMKG EFYVIEYAAC DATYNEIVTF ERLRPVNQNK TVKKNTFFKC TVDVPEDLRE ACANENAHKD FKKAVGACRI FYHPETTQLM ILSASEATVK RVNILSDMHL RSIRTKLMLM SRNEEATKHL ECTKQLAAAF HEEFVVREDL MGLAIGTHGS NIQQARKVPG VTAIELDEDT GTFRIYGESA DAVKKARGFL EFVEDFIQVP RNLVGKVIGK NGKVIQEIVD KSGVVRVRIE GDNENKLPRE DGMVPFVFVG TKESIGNVQV LLEY (1-353 aa encoded by BC028983)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.35 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 13194-1-AP (FXR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13194-1-AP (FXR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13194-1-AP ( FXR1 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 13194-1-AP (FXR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse heart tissue were subjected to SDS PAGE followed by western blot with 13194-1-AP (FXR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 13194-1-AP (FXR1 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Ethacrynic acid treated HepG2 cells using 13194-1-AP (FXR1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IGG.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Ethacrynic acid treated HepG2 cells using 13194-1-AP (FXR1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IGG.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using 13194-1-AP (FXR1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 13194-1-AP (FXR1 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 13194-1-AP (FXR1 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 13194-1-AP (FXR1 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 13194-1-AP (FXR1 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 13194-1-AP (FXR1 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 13194-1-AP (FXR1 antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-FXR1 (IP:13194-1-AP, 3ug; Detection:13194-1-AP 1:800) with mouse skeletal muscle tissue lysate 8000ug.