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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- GTX109198 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX109198, RRID:AB_10729461
- Product name
- EN2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Hsa-miR-27b is up-regulated in cytomegalovirus-infected human glioma cells, targets engrailed-2 and inhibits its expression.
Wang L, Yang M, Liao S, Liu W, Dai G, Wu G, Chen L
Experimental biology and medicine (Maywood, N.J.) 2017 Jun;242(12):1227-1233
Experimental biology and medicine (Maywood, N.J.) 2017 Jun;242(12):1227-1233
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: MCF-7 12% SDS PAGE GTX109198 diluted at 1:10000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- EN2 antibody immunoprecipitates EN2 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 £gg HeLa whole cell lysate/extract B. Control with 2 £gg of preimmune rabbit IgG C. Immunoprecipitation of EN2 protein by 2 £gg of EN2 antibody (GTX109198) 12% SDS-PAGE The immunoprecipitated EN2 protein was detected by EN2 antibody (GTX109198) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
