PA5-14363

antibody from Invitrogen Antibodies

Targeting: EN2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-14363

Provider

Invitrogen Antibodies

Product name

EN2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with chicken, Xenopus and zebrafish based on sequence homology.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Dual analysis of the murine cytomegalovirus and host cell transcriptomes reveal new aspects of the virus-host cell interface.

Juranic Lisnic V, Babic Cac M, Lisnic B, Trsan T, Mefferd A, Das Mukhopadhyay C, Cook CH, Jonjic S, Trgovcich J
PLoS pathogens 2013;9(9):e1003611

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis using an EN2 polyclonal antibody (Product # PA5-14363) in 293 cell lysates and mouse stomach and lung tissue lysates (35 µg per lane).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells stained with an EN2 polyclonal antibody (Product # PA5-14363). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with an EN2 polyclonal antibody (Product # PA5-14363) (1:25, 1 hr at 37°C). Primary antibody was detected with fluor-conjugated donkey anti-rabbit secondary antibody (green) at 1:400 dilution for 50 min at 37°C). Actin filaments have been labeled with dye-conjugated phalloidin (red). Nuclei were counterstained with DAPI (blue) (10 µg/mL, 10 min).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6 Validation of RNA-Seq analysis of host genes by western blot. (A) Immunoblot analysis of MEF.K (A) or Balb/c MEF (B-C) cell lysates infected with wild-type MCMV. Cell lysates were separated by SDS-PAGE, transferred to PVDF membrane, and probed with antibody to Jag2 (A), EN2 (B) or Trim71 (C). Monoclonal antibody to actin was used as loading control. Bar charts represent relative quantification of proteins. In the case of Trim71 (C where anti-Trim71 antibody detected multiple bands, the bars show quantification of the middle band.