GTX114650

Yoon SJ, Elahi LS, Pașca AM, Marton RM, Gordon A, Revah O, Miura Y, Walczak EM, Holdgate GM, Fan HC, Huguenard JR, Geschwind DH, Pașca SP
Nature methods 2019 Jan;16(1):75-78

Discovery of novel determinants of endothelial lineage using chimeric heterokaryons.

Wong WT, Matrone G, Tian X, Tomoiaga SA, Au KF, Meng S, Yamazoe S, Sieveking D, Chen K, Burns DM, Chen JK, Blau HM, Cooke JP
eLife 2017 Mar 21;6

DNA-PKcs, ATM, and ATR Interplay Maintains Genome Integrity during Neurogenesis.

Enriquez-Rios V, Dumitrache LC, Downing SM, Li Y, Brown EJ, Russell HR, McKinnon PJ
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Jan 25;37(4):893-905

Polynucleotide kinase-phosphatase enables neurogenesis via multiple DNA repair pathways to maintain genome stability.

Shimada M, Dumitrache LC, Russell HR, McKinnon PJ
The EMBO journal 2015 Oct 1;34(19):2465-80

Insulinoma-Associated Protein 1 Is a Crucial Regulator of Neuroendocrine Differentiation in Lung Cancer.

Fujino K, Motooka Y, Hassan WA, Ali Abdalla MO, Sato Y, Kudoh S, Hasegawa K, Niimori-Kita K, Kobayashi H, Kubota I, Wakimoto J, Suzuki M, Ito T
The American journal of pathology 2015 Dec;185(12):3164-77

Impact of Gender in Renal Cell Carcinoma: The Relationship of FABP7 and BRN2 Expression with Overall Survival.

Tan C, Takayama T, Takaoka N, Fujita H, Miyazaki M, Sugiyama T, Ozono S
Clinical Medicine Insights. Oncology 2014;8:21-7

Neurogenesis requires TopBP1 to prevent catastrophic replicative DNA damage in early progenitors.

Lee Y, Katyal S, Downing SM, Zhao J, Russell HR, McKinnon PJ
Nature neuroscience 2012 Jun;15(6):819-26

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Supportive validation

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Main image
Experimental details: Brn2 antibody detects POU3F2 protein by Western blot analysis.A. 30 µg A375 whole cell lysate/extract10 % SDS-PAGEBrn2 antibody (GTX114650) dilution: 1:1000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Whole cell extract (30 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with Brn2 antibody (GTX114650) diluted at 1:1000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Brn2 antibody detects Brn2 protein at nucleus by immunofluorescent analysis.Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Brn2 protein stained by Brn2 antibody (GTX114650) diluted at 1:1000.Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.Scale bar = 10 £gm.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Brn2 antibody immunoprecipitates Brn2 protein in IP experiments. IP Sample: 1000 ?g 293T whole cell lysate/extract A. 50 £gg 293T whole cell lysate/extract B. Control with 2 £gg of preimmune rabbit IgG C. Immunoprecipitation of Brn2 protein by 2 £gg of Brn2 antibody (GTX114650) 10% SDS-PAGE The immunoprecipitated Brn2 protein was detected by Brn2 antibody (GTX114650) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Supportive validation

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Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using Brn2(GTX114650) antibody at 1:500 dilution.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Brn2 antibody detects Brn2 protein expression at nucleus by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: Brn2 protein stained by Brn2 antibody (GTX114650) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Brn2 antibody detects Brn2 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Brn2 antibody (GTX114650) diluted at 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Brn2 antibody detects Brn2 protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Brn2 antibody (GTX114650) diluted at 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Brn2 antibody immunoprecipitates Brn2 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 £gg preimmune rabbit IgG B. 5 £gg of Brn2 antibody (GTX114650) The precipitated DNA was detected by PCR with primer set targeting to MITF promoter.