NBP2-61436

antibody from Novus Biologicals

Targeting: POU3F2 BRN2, OCT7, OTF7, POUF3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-61436

Provider

Novus Biologicals

Product name

Mouse Monoclonal POU3F2/OCT7 Antibody

Antibody type

Monoclonal

Description

Protein A purified. Specificity of human, mouse, rat POU3F2/OCT7 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Vial size

100 ul

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: POU3F2/OCT7 Antibody (CL6228) [NBP2-61436] - Analysis in human cerebral cortex tissue.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: POU3F2/OCT7 Antibody (CL6228) [NBP2-61436] - Analysis in mouse cerebral cortex tissue.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: POU3F2/OCT7 Antibody (CL6228) [NBP2-61436] - Analysis in human cell line U-251 MG.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: POU3F2/OCT7 Antibody (CL6228) [NBP2-61436] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-POU3F2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody (CL6228) [NBP2-61436] - Staining of mouse forebrain shows nuclear immunoreactivity in a subset of cells in the lateral ventricle wall.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody (CL6228) [NBP2-61436] - Staining of mouse cerebral cortex shows absence of positivity in layer 6 neurons as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody (CL6228) [NBP2-61436] - Staining of mouse cerebral cortex shows moderate nuclear positivity in neurons in layers 2-4.