AMAb91406

antibody from Atlas Antibodies

Targeting: POU3F2 BRN2, OCT7, OTF7, POUF3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AMAb91406

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#AMAb91406, RRID:AB_2716676

Product name

Anti-POU3F2

Antibody type

Monoclonal

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

ASNHYSLLTSSASIVHAEPPGGMQQGAGGYREAQS
LVQGDYGALQSNGHP


Isotype

IgG

Antibody clone number

CL6228

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cerebral cortex tissue.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line U-251 MG.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in mouse cerebral cortex tissue.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of U-251 cells using the Anti-POU3F2 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of mouse cerebral cortex shows moderate nuclear positivity in neurons in layers 2-4.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of mouse cerebral cortex shows absence of positivity in layer 6 neurons as expected.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of mouse forebrain shows nuclear immunoreactivity in a subset of cells in the lateral ventricle wall.