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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Chromatin Immunoprecipitation [1]
- Other assay [1]
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- Product number
- PA5-30124 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POU3F2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A375.
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Brn2 using 30 µg A375 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Brn2 polyclonal antibody (Product # PA5-30124) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of POU3F2 was performed by separating 30 µg of Whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a POU3F2 Polyclonal Antibody (Product # PA5-30124) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of POU3F2 was performed in SK-N-AS cells fixed in 4% paraformaldehyde at RT for 15 min. Green: POU3F2 Polyclonal Antibody (Product # PA5-30124) diluted at 1:1000. Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of POU3F2 was performed in paraffin-embedded mouse brain tissue using POU3F2 Polyclonal Antibody (Product # PA5-30124) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of POU3F2 was performed in paraffin-embedded rat brain tissue using POU3F2 Polyclonal Antibody (Product # PA5-30124) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of POU3F2 was performed in frozen sectioned E13.5 Rat brain tissue using POU3F2 Polyclonal Antibody (Product # PA5-30124) at a dilution of 1:250 (Green). Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using Brn2 (Product # PA5-30124) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Brn2 antibody immunoprecipitates Brn2 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract. A: 5 µg preimmune rabbit IgG. B: 5 µg of Brn2 antibody (Product # PA5-30124). The precipitated DNA was detected by PCR with primer set targeting to MITF promoter.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- POU3F2 Polyclonal Antibody immunoprecipitates Brn2 protein in IP experiments. IP Sample: 1,000 µg 293T whole cell lysate/extract A. 50 µg 293T whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of Brn2 protein by 2 µg of POU3F2 Polyclonal Antibody (Product # PA5-30124) 10% SDS-PAGE The immunoprecipitated Brn2 protein was detected by POU3F2 Polyclonal Antibody (Product # PA5-30124) diluted at 1:1,000.
