11674-1-AP

antibody from Invitrogen Antibodies

Targeting: PDLIM1 CLIM1, CLP-36, CLP36, hCLIM1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

11674-1-AP

Provider

Invitrogen Antibodies

Product name

PDLIM1/CLP36 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MTTQQIDLQ GPGPWGFRLV GGKDFEQPLA ISRVTPGSKA ALANLCIGDV ITAIDGENTS NMTHLEAQNR IKGCTDNLTL TVARSEHKVW SPLVTEEGKR HPYKMNLASE PQEVLHIGSA HNRSAMPFTA SPASSTTARV ITNQYNNPAG LYSSENISNF NNALESKTAA SGVEANSRPL DHAQPPSSLV IDKESEVYKM LQEKQELNEP PKQSTSFLVL QEILESEEKG DPNKPSGFRS VKAPVTKVAA SIGNAQKLPM CDKCGTGIVG VFVKLRDRHR HPECYVCTDC GTNLKQKGHF FVEDQIYCEK HARERVTPPE GYEVVTVFPK (1-329 aa encoded by BC000915)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.6 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human lung tissue were subjected to SDS PAGE followed by western blot with 11674-1-AP (PDLIM1/CLP36 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human ileum tissue were subjected to SDS PAGE followed by western blot with 11674-1-AP (PDLIM1/CLP36 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse heart tissue were subjected to SDS PAGE followed by western blot with 11674-1-AP (PDLIM1/CLP36 antibody at dilution of 1:600 incubated at 4 degree celsius over night.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse lung tissue were subjected to SDS PAGE followed by western blot with 11674-1-AP ( PDLIM1/CLP36 antibody at dilution of 1:600 incubated at 4 degree celsius over night.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells, using PDLIM1 antibody 11674-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas cancer using 11674-1-AP (PDLIM1/CLP36 antibody) at dilution of 1:50 (under 10x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 MCF-7 cells were stained with 0.2ug PDLIM1/CLP36 antibody (11674-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PDLIM1/CLP36 (IP:11674-1-AP, 3ug; Detection:11674-1-AP 1:600) with mouse heart tissue lysate 4000ug.