GTX130536

antibody from GeneTex

Targeting: DICER1 Dicer, HERNA, K12H4.8-LIKE, KIAA0928, MNG1

Western blot (Enhanced validation)

Antibody Data

Product number

GTX130536

Provider

GeneTex

Product name

Dicer antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

Western blot

Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Non-transfected (¡V) and transfected (+) MCF-7 whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody (GTX130536) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

KD Reagent Type

siRNA

Downregulation

>50%

KD Reagent Provider

shRNA reagents was provided courtesy of cooperative labs.

KD Reagent Prod no

Proprietary.

KD Reagent Prod page

KD Reagent Prod page

Antibody Lot Number

41983

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Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Non-transfected (¡V) and transfected (+) SW480 whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody (GTX130536) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

KD Reagent Type

siRNA

Downregulation

>50%

KD Reagent Prod page

KD Reagent Prod page

KD Reagent Prod no

Proprietary.

KD Reagent Provider

shRNA reagents was provided courtesy of cooperative labs.

Antibody Lot Number

41983

Show more

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Dicer antibody detects Dicer protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody (GTX130536) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody (GTX130536) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) MCF-7 whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody (GTX130536) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) SW480 whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody (GTX130536) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.