H00005979-D01P

antibody from Abnova Corporation

Targeting: RET CDHF12, CDHR16, HSCR1, MEN2A, MEN2B, MTC1, PTC, RET51

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005979-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005979-D01P, RRID:AB_1707331

Product name

RET purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human RET protein.

Antigen sequence

MAKATSGAAGLRLLLLLLLPLLGKVALGLYFSRDA
YWEKLYVDQAAGTPLLYVHALRDAPEEVPSFRLGQ
HLYGTYRTRLHENNWICIQEDTGLLYLNRSLDHSS
WEKLSVRNRGFPLLTVYLKVFLSPTSLREGECQWP
GCARVYFSFFNTSFPACSSLKPRELCFPETRPSFR
IRENRPPGTFHQFRLLPVQFLCPNISVAYRLLEGE
GLPFRCAPDSLEVSTRWALDREQREKYELVAVCTV
HAGAREEVVMVPFPVTVYDEDDSAPTFPAGVDTAS
AVVEFKRKEDTVVATLRVFDADVVPASGELVRRYT
STLLPGDTWAQQTFRVEHWPNETSVQANGSFVRAT
VHDYRLVLNRNLSISENRTMQLAVLVNDSDFQGPG
AGVLLLHFNVSVLPVSLHLPSTYSLSVSRRARRFA
QIGKVCVENCQAFSGINVQYKLHSSGANCSTLGVV
TSAEDTSGILFVNDTKALRRPKCAELHYMVVATDQ
QTSRQAQAQLLVTVEGSYVAEEAGCPLSCAVSKRR
LECEECGGLGSPTGRCEWRQGDGKGITRNFSTCSP
STKTCPDGHCDVVETQDINICPQDCLRGSIVGGHE
PGEPRGIKAGYGTCNCFPEEEKCFCEPEDIQDPLC
DELCRTVIAAAVLFSFIVSVLLSAFCIHCYHKFAH
KPPISSAEMTFRRPAQAFPVSYSSSSARRPSLDSM
ENQVSVDAFKILEDPKWEFPRKNLVLGKTLGEGEF
GKVVKATAFHLKGRAGYTTVAVKMLKENASPSELR
DLLSEFNVLKQVNHPHVIKLYGACSQDGPLLLIVE
YAKYGSLRGFLRESRKVGPGYLGSGGSRNSSSLDH
PDERALTMGDLISFAWQISQGMQYLAEMKLVHRDL
AARNILVAEGRKMKISDFGLSRDVYEEDSYVKRSQ
GRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIV
TLGGNPYPGIPPERLFNLLKTGHRMERPDNCSEEM
YCLMLQCWKQEPDKRPVFADISKDLEKMMVKRRDY
LDLAASTPSDSLIYDDGLSEEETPLVDCNNAPLPR
ALPSTWIENKLYGRISHAFTRF


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

RET MaxPab rabbit polyclonal antibody. Western Blot analysis of RET expression in mouse liver.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of RET expression in transfected 293T cell line (H00005979-T02) by RET MaxPab polyclonal antibody.Lane 1: RET transfected lysate(119.80 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between RET and MAPK1. HeLa cells were stained with anti-RET rabbit purified polyclonal 1:1200 and anti-MAPK1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)