PA5-111870

antibody from Invitrogen Antibodies

Targeting: RET CDHF12, CDHR16, HSCR1, MEN2A, MEN2B, MTC1, PTC, RET51

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Immunoelectron microscopy (Recommended by provider)

Antibody Data

Product number

PA5-111870

Provider

Invitrogen Antibodies

Product name

RET (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

50 µL

Concentration

0.8 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RET was performed in rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes. Lane 1,2: RET (extracellular) Antibody (Product # PA5-111870) at a dilution of 1:200. Lane 3,4: RET (extracellular) Antibody preincubated with the negative control antigen.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of RET in live intact human MCF-7 breast adenocarcinoma cells. A) Extracellular staining of cells with RET (extracellular) Antibody (Product # PA5-111870), (1:25), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B) Live image of the cells. C) Merge of the two images.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RET in frozen rat DRG tissue sections using RET (extracellular) Antibody (Product # PA5-111870) at a dilution of 1:50. Ret staining (red) is detected in DRG neurons. Hoechst 33342 (blue) is used to stain cell nuclei.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of RET in live intact human THP-1 monocytic leukemia cell line. Black: Unstained cells and goat-anti-rabbit-AlexaFluor-647 secondary antibody. Green: Cells and RET (extracellular) Antibody (Product # PA5-111870), (1:20) and goat-anti-rabbit-AlexaFluor-647 secondary antibody.