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LearnANT-025-200UL
antibody from Invitrogen Antibodies
Targeting: RET
CDHF12, CDHR16, HSCR1, MEN2A, MEN2B, MTC1, PTC, RET51
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- ANT-025-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Ret (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Reconstitution: 1 X 25 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-Ret (extracellular) Antibody (#ANT-025), (1:200).3,4. Anti-Ret (extracellular) Antibody , preincubated with Ret (extracellular) Blocking Peptide (#BLP-NT025).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of Ret in human MCF-7 breast adenocarcinoma cells - Cell surface detection of Ret in live intact human MCF-7 breast adenocarcinoma cells. A. Extracellular staining of cells with Anti-Ret (extracellular) Antibody (#ANT-025), (1:25), followed by goat Anti-rabbit-AlexaFluor-594secondary Antibody (red). B. Live image of the cells. C. Merge of the two images.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of Ret in rat DRG - Immunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti-Ret (extracellular) Antibody (#ANT-025), (1:50). Ret staining (red) is detected in DRG neurons. Hoechst 33342 (blue) is used to stain cell nuclei.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of Ret by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-Ret (extracellular) Antibody (#ANT-025), (5μg) + goat- Anti-rabbit-FITC.
