Explore
Validate
LearnAF5009
antibody from R&D Systems
Targeting: RET
CDHF12, CDHR16, HSCR1, MEN2A, MEN2B, MTC1, PTC, RET51
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF5009 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Phospho-Ret (Y1062) Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Ret when phosphorylated at Y1062 in Western blots.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Mortalin (GRP75/HSPA9) upregulation promotes survival and proliferation of medullary thyroid carcinoma cells.
Endoneurial macrophages induce perineural invasion of pancreatic cancer cells by secretion of GDNF and activation of RET tyrosine kinase receptor.
Starenki D, Hong SK, Lloyd RV, Park JI
Oncogene 2015 Aug 27;34(35):4624-34
Oncogene 2015 Aug 27;34(35):4624-34
Endoneurial macrophages induce perineural invasion of pancreatic cancer cells by secretion of GDNF and activation of RET tyrosine kinase receptor.
Cavel O, Shomron O, Shabtay A, Vital J, Trejo-Leider L, Weizman N, Krelin Y, Fong Y, Wong RJ, Amit M, Gil Z
Cancer research 2012 Nov 15;72(22):5733-43
Cancer research 2012 Nov 15;72(22):5733-43
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Phospho-Ret (Y1062) by Western Blot. Western blot shows lysates of TT human medullary thyroid cancer cell line untreated (-) or treated (+) with 100 μM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Human Phospho-Ret (Y1062) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5009), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Ret (Y1062) at approximately 175 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
