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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- GTX113296 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX113296, RRID:AB_1952625
- Product name
- 14-3-3 beta antibody [N1C2]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (20 ug of whole cell lysate) A: mouse brain 12% SDS PAGE GTX113296 diluted at 1:10000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A431 (GTX27909) B: H1299 C: Hela D: Hep G2 (GTX27900) E: Molt-4 (GTX27912) F: Raji 12% SDS PAGE GTX113296 diluted at 1:10000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membranes were blotted with 14-3-3 beta antibody [N1C2] (GTX113296) diluted at 1:10000 and competitor's antibody (sc-628) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 14-3-3 beta (GTX113296) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using 14-3-3 beta(GTX113296) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- 14-3-3 beta antibody [N1C2] detects 14-3-3 beta protein at cytoplasm in rat cervix by immunohistochemical analysis. Sample: Paraffin-embedded rat cervix. 14-3-3 beta antibody [N1C2] (GTX113296) diluted at 1:500.
