PA1-4612

antibody from Invitrogen Antibodies

Targeting: YWHAB YWHAA

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-4612

Provider

Invitrogen Antibodies

Product name

Phospho-14-3-3 beta (Ser58) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with bovine, canine, chicken, mouse, non-human primate, sheep, Xenopus and zebrafish based on 100% sequence homology.

References

Submitted references

CCM3/PDCD10 stabilizes GCKIII proteins to promote Golgi assembly and cell orientation.

Fidalgo M, Fraile M, Pires A, Force T, Pombo C, Zalvide J
Journal of cell science 2010 Apr 15;123(Pt 8):1274-84

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Serine 58 of 14-3-3zeta is a molecular switch regulating ASK1 and oxidant stress-induced cell death.

Zhou J, Shao Z, Kerkela R, Ichijo H, Muslin AJ, Pombo C, Force T
Molecular and cellular biology 2009 Aug;29(15):4167-76

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of rat brainstem lysate showing specific immunolabeling of the ~29 kDa 14-3-3 protein phosphorylated at Ser58 (Lane 1). The labeling by Anti-Ser58 14-3-3 is specifically blocked by the Ser58 phosphopeptide (Lane 2) used as antigen. The corresponding non-phosphopeptide did not block the immunolabeling (not shown).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of 14-3-3 beta in rat brainstem lysate showing specific immunolabeling of a band at ~29 kDa corresponding to Phospho-14-3-3 beta (Ser58) polyclonal antibody (Product # PA1-4612) first lane (-). The immunolabeling is blocked by the phosphopeptide used as the antigen second lane (+) but not by the corresponding non-phosphopeptide (not shown).