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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-28112 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-14-3-3 beta Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA5-28112 targets 14-3-3 beta in IF, IHC (P), and WB applications and shows reactivity with Human samples. The PA5-28112 immunogen is synthetic peptide corresponding to a region within amino acids 89 and 183 of Human 14-3-3 beta.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 9.06 mg/ml
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of 14-3-3 beta using 30µg of Raji lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a 14-3-3 beta polyclonal antibody (Product # PA5-28112) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 14-3-3 beta in methanol-fixed HeLa cells using a 14-3-3 beta polyclonal antibody (Product # PA5-28112) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of 14-3-3 beta in paraffin-embedded Ca922 xenograft using a 14-3-3 beta polyclonal antibody (Product # PA5-28112) at a dilution of 1:500.
