AM09043PU-N

antibody from Acris Antibodies GmbH

Targeting: YWHAB YWHAA

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM09043PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM09043PU-N, RRID:AB_1609701

Product name

anti 14-3-3 protein beta/alpha

Antibody type

Monoclonal

Antigen

Recombinant human 14-3-3 beta (1-246 aa) purified from E. coli

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

J2E9

Vial size

0.1 ml

Concentration

1.0 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis: Cell lysates of HeLa (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-Human 14-3-3 beta antibody (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Cell lysates of HeLa(30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human 14-3-3 beta (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry: AM09043PU-N 14-3-3 beta antibody staining of Formalin-Fixed, Paraffin-Embedded Human Brain, Cortex at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry: AM09043PU-N 14-3-3 beta antibody staining of Formalin-Fixed, Paraffin-Embedded Human Kidney at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry: AM09043PU-N 14-3-3 beta antibody staining of Formalin-Fixed, Paraffin-Embedded Human Thyroid at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.