A01127

antibody from Boster Biological Technology

Targeting: SFN YWHAS

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

A01127

Provider

Boster Biological Technology

Product name

Anti-14-3-3 sigma/SFN Antibody Picoband™

Antibody type

Polyclonal

Description

Polyclonal antibody for STRATIFIN/SFN detection. Host: Rabbit.Size: 100μg/vial. Tested applications: ELISA. Reactive species: Human. STRATIFIN/SFN information: Subcellular Localization: Cytoplasm. Nucleus . Secreted. May be secreted by a non-classical secretory pathway; Tissue Specificity: Present mainly in tissues enriched in stratified squamous keratinizing epithelium.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of 14-3-3 sigma using anti-14-3-3 sigma antibody (A01127). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela cell lysate,Lane 2: human placenta tissue lysate,Lane 3: human MCF-7 cell lysate,Lane 4: human 22RV1 cell lysate,Lane 5: rat lung tissue lysate,Lane 6: rat PC-12 cell lysate,Lane 7: mouse lung tissue lysate,Lane 8: mouse HEPA1-6 cell lysate,Lane 9: mouse NIH3T3 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-14-3-3 sigma antigen affinity purified polyclonal antibody (Catalog # A01127) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 14-3-3 sigma at approximately 28KD. The expected band size for 14-3-3 sigma is at 28KD.

Additional image