Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 11-1020 - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-14-3-3 sigma Antibody
- Antibody type
- Polyclonal
- Description
- 14-3-3 sigma is a member of a highly conserved family of 14-3-3 proteins that are present in all eukaryotic organisms. There are 7 known human 14-3-3 isotypes and they play important roles in many biological activities by binding to and altering the subcellular localization and/or stability of key molecules in various signaling cascades. 14-3-3 sigma was originally characterized as a human mammary epithelium marker 1 and later rediscovered as an important molecule for cell cycle checkpoint regulation. Recently it has been reported that 14-3-3 sigma may serve as a prognosis marker predicting survival of pancreatic cancer patient treatment. 14-3-3 Sigma is the only 14-3-3 isoform induced by the tumor suppressor protein p53, in response to Gamma-irradiation and other DNA-damaging agents. 14-3-3 sigma is a p53-regulated inhibitor of G2/M progression and acts as a tumor suppressor gene that is inactivated by methylation of its 5' CpG islands in epithelial tumor cells. Two isoforms of the human proteins are produced by alternative splicing.
- Reactivity
- Human
- Host
- Rabbit
- Antigen sequence
Full length recombinant 14-3-3 sigm
a protein was used as the immunogen
for this antibody.- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freezing and thawing
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Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of 14-3-3 sigma . Anti- 14-3-3 sigma antibody (11-1020) was used at 1 µg/ml on Molt-4 lysate.
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of 14-3-3 sigma. Anti-14-3-3 sigma antibody (11-1020) in human Colon tissue at 10 µg/ml.
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of 14-3-3 sigma. Anti-14-3-3 sigma antibody (11-1020) in human Skin tissue at 10 µg/ml.