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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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- Product number
- GTX113466 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX113466, RRID:AB_2037194
- Product name
- HPRT antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Critical involvement of ZEB2 in collagen fibrillogenesis: the molecular similarity between Mowat-Wilson syndrome and Ehlers-Danlos syndrome.
Teraishi M, Takaishi M, Nakajima K, Ikeda M, Higashi Y, Shimoda S, Asada Y, Hijikata A, Ohara O, Hiraki Y, Mizuno S, Fukada T, Furukawa T, Wakamatsu N, Sano S
Scientific reports 2017 Apr 19;7:46565
Scientific reports 2017 Apr 19;7:46565
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: Molt-4 (GTX27912) B: Raji 12% SDS PAGE GTX113466 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HPRT antibody detects HPRT1 protein by western blot analysis.A. 50 ?g mouse liver lysate/extract12% SDS-PAGEHPRT antibody (GTX113466) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HPRT antibody detects HPRT protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody (GTX113466) diluted by 1:1000.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody (GTX113466) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody (GTX113466) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HPRT(GTX113466) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HPRT antibody detects HPRT protein at cytoplasm in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. HPRT antibody (GTX113466) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HPRT antibody detects HPRT protein at cytoplasm in human endometrial cancer by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial cancer. HPRT antibody (GTX113466) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HPRT antibody detects HPRT protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. HPRT antibody (GTX113466) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HPRT antibody detects HPRT protein at cytoplasm in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. HPRT antibody (GTX113466) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HPRT antibody detects HPRT protein at cytoplasm in rat colon by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. HPRT antibody (GTX113466) diluted at 1:500.
