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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB12241 - Provider product page
- Provider
- Abnova Corporation
- Product name
- HPRT1 monoclonal antibody, clone 5F11A7
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against full length recombinant human HPRT1.
- Isotype
- IgG
- Antibody clone number
- 5F11A7
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of human HPRT1 (AA:1-218) recombinant protein (Expected MW is 50.5 kDa) with HPRT1 monoclonal antibody, clone 5F11A7 (Cat# MAB12241) at 1:500-1:2000 dilution.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of Lane 1: Hela cell lysate; Lane 2: A431 cell lysate; Lane 3: A549 cell lysate with HPRT1 monoclonal antibody, clone 5F11A7 (Cat# MAB12241) at 1:500-1:2000 dilution.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [HPRT1 (AA: 1-218)-hIgGFc transfected HEK293 cells] with HPRT1 monoclonal antibody, clone 5F11A7 (Cat# MAB12241) at 1:500-1:2000 dilution.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA analysis of HPRT1 monoclonal antibody, clone 5F11A7 (Cat# MAB12241) at 1:10000 dilution.
- Validation comment
- ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human kidney tissues with HPRT1 monoclonal antibody, clone 5F11A7 (Cat# MAB12241) at 1:200-1:1000 dilution.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of Hela cells with HPRT1 monoclonal antibody, clone 5F11A7 (Cat# MAB12241) at 1:200-1:400 dilution (Green) and negative control (Red).
- Validation comment
- Flow Cytometry
