15059-1-AP

antibody from Invitrogen Antibodies

Targeting: HPRT1 HGPRT, HPRT

Immunoprecipitation

Antibody data

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Validation data

Product number: 15059-1-AP - Provider product page
Provider: Invitrogen Antibodies
Product name: HPRT1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Other
Description: Immunogen sequence: MATRSPGVV ISDDEPGYDL DLFCIPNHYA EDLERVFIPH GLIMDRTERL ARDVMKEMGG HHIVALCVLK GGYKFFADLL DYIKALNRNS DRSIPMTVDF IRLKSYCNDQ STGDIKVIGG DDLSTLTGKN VLIVEDIIDT GKTMQTLLSL VRQYNPKMVK VASLLVKRTP RSVGYKPDFV GFEIPDKFVV GYALDYNEYF RDLNHVCVIS ETGKAKYKA (1-218 aa encoded by BC000578 )
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 150 µL
Concentration: 0.29 mg/mL
Storage: -20°C

HPRT1 protein structure - 15059-1-AP shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HepG2 cells were subjected to SDS PAGE followed by western blot with 15059-1-AP (HPRT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Various lysates were subjected to SDS PAGE followed by western blot with 15059-1-AP (HPRT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: MCF7 cells were subjected to SDS PAGE followed by western blot with 15059-1-AP (HPRT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: A549 cells were subjected to SDS PAGE followed by western blot with 15059-1-AP (HPRT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Mouse liver tissue were subjected to SDS PAGE followed by western blot with 15059-1-AP (HPRT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Rat brain tissue were subjected to SDS PAGE followed by western blot with 15059-1-AP (HPRT1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 15059-1-AP (HPRT1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 15059-1-AP (HPRT1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human pancreas slide using 15059-1-AP (HPRT1 Antibody) at dilution of 1:50.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human pancreas slide using 15059-1-AP (HPRT1 Antibody) at dilution of 1:50.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human brain using 15059-1-AP (HPRT1 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human brain using 15059-1-AP (HPRT1 antibody) at dilution of 1:100 (under 40x lens).