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ImmunocytochemistryAntibody data
- Antibody Data
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- References [1]
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- Western blot [5]
- Immunohistochemistry [5]
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- Product number
- NBP1-33527 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-33527, RRID:AB_2120558
- Product name
- Rabbit Polyclonal HPRT Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Hypoxia drives the assembly of the multienzyme purinosome complex.
Doigneaux C, Pedley AM, Mistry IN, Papayova M, Benkovic SJ, Tavassoli A
The Journal of biological chemistry 2020 Jul 10;295(28):9551-9566
The Journal of biological chemistry 2020 Jul 10;295(28):9551-9566
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HPRT Antibody [NBP1-33527] - Sample (30 ug of whole cell lysate) A: Molt-4 B: Raji 12% SDS PAGE; antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HPRT Antibody [NBP1-33527] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HPRT Antibody [NBP1-33527] - A. 50 ug mouse liver lysate/extract 12 % SDS-PAGE HPRT antibody dilution: 1:500
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HPRT Antibody [NBP1-33527] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HPRT Antibody [NBP1-33527] - Wild-type (WT) and HPRT knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-33527] - Paraffin-embedded human endometrial cancer. HPRT antibody) diluted at 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-33527] - Paraffin-embedded human colon cancer. HPRT antibody diluted at 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-33527] - Paraffin-embedded rat brain. HPRT antibody diluted at 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-33527] - Paraffin-embedded mouse colon. HPRT antibody diluted at 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-33527] - Paraffin-embedded rat colon. HPRT antibody diluted at 1:500.
