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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
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- Product number
- PA5-95780 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HEBP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MAEPLQPDPG AAEDAAAQAV ETPGWKAPED AGPQPGSYEI RHYGPAKWVS TSVESMDWDS AIQTGFTKLN SYIQGKNEKE MKIKMTAPVT SYVEPGSGPF SESTITISLY IPSEQQFDPP RPLESDVFIE DRAEMTVFVR SFDGFSSAQK NQEQLLTLAS ILREDGKVFD EKVYYTAGYN SPVKLLNRNN EVWLIQKNEP TKENE; Positive Samples: COS-7, Mouse lung; Cellular Location: Cytoplasm, Mitochondrion
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.33 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of HEBP2 in extracts of various cell lines using HEBP2 Polyclonal Antibody (Product # PA5-95780) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of HEBP2 was performed in C6 cells using HEBP2 Polyclonal Antibody (Product # PA5-95780) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of HEBP2 was performed in L929 cells using HEBP2 Polyclonal Antibody (Product # PA5-95780) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of HEBP2 was performed in U-2 OS cells using HEBP2 Polyclonal Antibody (Product # PA5-95780) at a dilution of 1:100. Blue: DAPI for nuclear staining.
