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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- RQ5974 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- EPS15 Antibody / Epidermal growth factor receptor substrate 15
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Storage
- After reconstitution, the EPS15 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat brain, 2) mouse brain, 3) mouse NIH 3T3 and human 4) HeLa, 5) Jurkat, 6) HEK293, 7) PC-3 and 8) ThP1 lysate with EPS15 antibody. Predicted molecular weight ~99 kDa, routinely observed at 120-150 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human A431 cells with EPS15 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= EPS15 antibody.
