H00006610-M01

antibody from Abnova Corporation

Targeting: SMPD2 ISC1, nSMase

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00006610-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00006610-M01, RRID:AB_463927

Product name

SMPD2 monoclonal antibody (M01), clone 2C9

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant SMPD2.

Antigen sequence

MKPNFSLRLRIFNLNCWGIPYLSKHRADRMRRLGD
FLNQESFDLALLEEVWSEQDFQYLRQKLSPTYPAA
HHFRSGIIGSGLCVFSKHPIQELTQHIYTLNGYPY
MIHHGDWFSGKAVGLLVLHLSGMVLNAYVTHLHAE
YNRQKDIYLAHRVAQAWELAQFIHHTSKKADVVLL
CGDLNMHPEDLGCCLLKEWTGLHDAYLETRDFKGS
EEGNTMVPKNCYVSQQELKPFPFGVRIDYVLYKAV
SGFYISCKSFETTTGFDPHSGTPLSDHEALMATLF
VRHSPPQQNPSSTHGPAERSPLMCVLKEAWTELGL
GMAQARWWATFASYVIGLGLLLLALLCVLAAGGGA
GEAAILLWTPSVGLVLWAGAFYLFHVQEVNGLYRA
QAELQHVLGRAREAQDLGPEPQPALLLGQQEGDRT
KEQ


Isotype

IgG

Antibody clone number

2C9

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Dysregulated sphingolipid metabolism in endometriosis.

Lee YH, Tan CW, Venkatratnam A, Tan CS, Cui L, Loh SF, Griffith L, Tannenbaum SR, Chan JK
The Journal of clinical endocrinology and metabolism 2014 Oct;99(10):E1913-21

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged SMPD2 is approximately 10ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to SMPD2 on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol