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- Antigen structure
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- Western blot [1]
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- Product number
- MAB7097 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Pannexin-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Pannexin-1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Pannexin-2 or -3 is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q96RD7- Isotype
- IgG
- Antibody clone number
- 720505
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Novel evidence that extracellular nucleotides and purinergic signaling induce innate immunity-mediated mobilization of hematopoietic stem/progenitor cells.
Human stem cells express pannexins.
Adamiak M, Bujko K, Cymer M, Plonka M, Glaser T, Kucia M, Ratajczak J, Ulrich H, Abdel-Latif A, Ratajczak MZ
Leukemia 2018 Sep;32(9):1920-1931
Leukemia 2018 Sep;32(9):1920-1931
Human stem cells express pannexins.
Hainz N, Beckmann A, Schubert M, Haase A, Martin U, Tschernig T, Meier C
BMC research notes 2018 Jan 22;11(1):54
BMC research notes 2018 Jan 22;11(1):54
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat Pannexin-1 by Western Blot. Western blot shows lysates of human brain tissue, mouse brain tissue, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Pannexin-1 Monoclonal Antibody (Catalog # MAB7097) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Pannexin-1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
