PAB21861

antibody from Abnova Corporation

Targeting: PRDM10 KIAA1231, MGC131802, PFM7

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB21861

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB21861, RRID:AB_10987164

Product name

PRDM10 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant PRDM10.

Antigen sequence

SPSHIQGSSSTQGQALQQQQQQQQNSSVQHTYLPS
AWNSFRGYSSEIQMMTLPPGQFVITDSGVATPVTT
GQVKAVTSGHYVLSESQSELEEKQTSALSGGVQVE
PPAHSDSLDPQTNSQQQTTQYIITTTTNGNGS


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with PRDM10 polyclonal antibody (Cat # PAB21861) at 1:250-1:500 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human duodenum with PRDM10 polyclonal antibody (Cat # PAB21861) shows strong nuclear positivity in glandular cells while paneth cells displayed additional strong cytoplasmic positivity at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)