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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- NB300-889 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-889, RRID:AB_10001047
- Product name
- Goat Polyclonal ADAM12 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise both the longer membrane-bound form of human ADAM12 (NP_003465.3) and the shorter soluble ADAM12 splice isoform (NP_067673.2). This antibody does not cross-react with other ADAMS.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references ADAM-10 is overexpressed in rheumatoid arthritis synovial tissue and mediates angiogenesis.
Binding of ADAM12, a marker of skeletal muscle regeneration, to the muscle-specific actin-binding protein, alpha -actinin-2, is required for myoblast fusion.
Binding of ADAM12, a marker of skeletal muscle regeneration, to the muscle-specific actin-binding protein, alpha -actinin-2, is required for myoblast fusion.
Isozaki T, Rabquer BJ, Ruth JH, Haines GK 3rd, Koch AE
Arthritis and rheumatism 2013 Jan;65(1):98-108
Arthritis and rheumatism 2013 Jan;65(1):98-108
Binding of ADAM12, a marker of skeletal muscle regeneration, to the muscle-specific actin-binding protein, alpha -actinin-2, is required for myoblast fusion.
Galliano MF, Huet C, Frygelius J, Polgren A, Wewer UM, Engvall E
The Journal of biological chemistry 2000 May 5;275(18):13933-9
The Journal of biological chemistry 2000 May 5;275(18):13933-9
Binding of ADAM12, a marker of skeletal muscle regeneration, to the muscle-specific actin-binding protein, alpha -actinin-2, is required for myoblast fusion.
Galliano MF, Huet C, Frygelius J, Polgren A, Wewer UM, Engvall E
The Journal of biological chemistry 2000 May 5;275(18):13933-9
The Journal of biological chemistry 2000 May 5;275(18):13933-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ADAM12 Antibody [NB300-889] - Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ADAM12 Antibody [NB300-889] - Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: ADAM12 Antibody [NB300-889] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
