PA1-84881

antibody from Invitrogen Antibodies

Targeting: ADIPOQ ACDC, ACRP30, adiponectin, apM1, GBP28

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-84881

Provider

Invitrogen Antibodies

Product name

Adiponectin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. In Western blot, this antibody detects a band at ~26.4kDa in human HL60 cell lysates. A suggested positive control for PA1-84881 is rat brain.

Concentration

1 mg/mL

References

Submitted references

Serum concentration and vascular expression of adiponectin are differentially associated with the diabetic calcifying peripheral arteriopathy.

Aubert CE, Liabeuf S, Amouyal C, Kemel S, Lajat-Kiss F, Lacorte JM, Halbron M, Carlier A, Salem JE, Funck-Brentano C, Perisic Matic L, Witasp A, Stenvinkel P, Phan F, Massy ZA, Hartemann A, Bourron O
Diabetology & metabolic syndrome 2019;11:32

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of whole cell lysate from HL60 promyelocytic leukemia cells probed with a Adiponectin polyclonal antibody (Product # PA1-84881) at 0.5 (A), 1 (B) and 2 (C) µg/mL

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical staining of HL60 cells using a Adiponectin polyclonal antibody (Product # PA1-84881).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of rat brain using a Adiponectin polyclonal antibody (Product # PA1-84881)

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 3 Immunohistochemical staining of adiponectin in arteries from patients with type 2 diabetes. Adiponectin (ADIPOQ) protein expression and localization was examined by immunohistochemistry staining in arteries from diabetic patients, classified according to the grade of calcification. ADIPOQ (red signal) was found to be expressed in all stages of calcification, but it was particularly enriched in early stages (grade 1). Smooth muscle cells of the media were specifically immuno positive for ADIPOQ, but the signal was also present in microvessels of the adventitia (particularly grade 2). Images were taken with x5 magnification and enlarged images with x15 (size bars included)