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ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- ELISA [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00003556-M06 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00003556-M06, RRID:AB_1576282
- Product name
- IL1RAP monoclonal antibody (M06), clone 2A3
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant IL1RAP.
- Antigen sequence
MTLLWCVVSLYFYGILQSDASERCDDWGLDTMRQI
QVFEDEPARIKCPLFEHFLKFNYSTAHSAGLTLIW
YWTRQDRDLEEPINFRLPENRISKEKDVLWFRPTL
LNDTGNYTCMLRNTTYCSKVAFPLEVVQKDSCFNS
PMKLPVHKLYIEYGIQRITCPNVDGYFPSSVKPTI
TWYMGCYKIQNFNNVIPEGMNLSFLIALISNNGNY
TCVVTYPENGRTFHLTRTPTVKVVGSPKNAVPPVI
HSPNDHVVYEKEPGEELLIPCTVYFSFLMDSRNEV
WWTIDGKKPDDITIDVTINESISHSRTEDETRTQI
LSIKKVTSEDLKRSYVCHARSAKGEVAKAAKVKQK
GNRCGQ- Isotype
- IgG
- Antibody clone number
- 2A3
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged IL1RAP is 0.3 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of IL1RAP transfected lysate using anti-IL1RAP monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with IL1RAP MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
