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- Antibody Data
- Antigen structure
- References [4]
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- Western blot [1]
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- Product number
- MAB10311 - Provider product page
- Provider
- R&D Systems
- Product name
- Human ADAM8 aa 498-653 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human ADAM8 in direct ELISAs and Western blots. Detects aa 498-653 of rhADAM8, which corresponds to the cysteine-rich and EGF-like domains. In direct ELISAs, no cross-reactivity with recombinant human (rh) ADAM9, rhADAM10, rhADAM15, rhBACE, or rhTACE is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P78325- Isotype
- IgG
- Antibody clone number
- 143338
- Vial size
- 500 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references miR-720 is a downstream target of an ADAM8-induced ERK signaling cascade that promotes the migratory and invasive phenotype of triple-negative breast cancer cells.
Expression and regulation of the metalloproteinase ADAM-8 during human neutrophil pathophysiological activation and its catalytic activity on L-selectin shedding.
Expression and regulation of the metalloproteinase ADAM-8 during human neutrophil pathophysiological activation and its catalytic activity on L-selectin shedding.
Eosinophils adhere to vascular cell adhesion molecule-1 via podosomes.
Das SG, Romagnoli M, Mineva ND, Barillé-Nion S, Jézéquel P, Campone M, Sonenshein GE
Breast cancer research : BCR 2016 Apr 2;18(1):40
Breast cancer research : BCR 2016 Apr 2;18(1):40
Expression and regulation of the metalloproteinase ADAM-8 during human neutrophil pathophysiological activation and its catalytic activity on L-selectin shedding.
Gómez-Gaviro M, Domínguez-Luis M, Canchado J, Calafat J, Janssen H, Lara-Pezzi E, Fourie A, Tugores A, Valenzuela-Fernández A, Mollinedo F, Sánchez-Madrid F, Díaz-González F
Journal of immunology (Baltimore, Md. : 1950) 2007 Jun 15;178(12):8053-63
Journal of immunology (Baltimore, Md. : 1950) 2007 Jun 15;178(12):8053-63
Expression and regulation of the metalloproteinase ADAM-8 during human neutrophil pathophysiological activation and its catalytic activity on L-selectin shedding.
Gómez-Gaviro M, Domínguez-Luis M, Canchado J, Calafat J, Janssen H, Lara-Pezzi E, Fourie A, Tugores A, Valenzuela-Fernández A, Mollinedo F, Sánchez-Madrid F, Díaz-González F
Journal of immunology (Baltimore, Md. : 1950) 2007 Jun 15;178(12):8053-63
Journal of immunology (Baltimore, Md. : 1950) 2007 Jun 15;178(12):8053-63
Eosinophils adhere to vascular cell adhesion molecule-1 via podosomes.
Johansson MW, Lye MH, Barthel SR, Duffy AK, Annis DS, Mosher DF
American journal of respiratory cell and molecular biology 2004 Oct;31(4):413-22
American journal of respiratory cell and molecular biology 2004 Oct;31(4):413-22
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human ADAM8 by Western Blot. Western blot shows Recombinant Human ADAM8 aa158-653 and Recombinant Human ADAM8 aa 158-497. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human ADAM8 Monoclonal Antibody (Catalog # MAB10311) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for ADAM8 aa158-653 at approximately 65 kDa (as indicated). The antibody did not detect ADAM8 aa 158-497, indicating it is specific for aa 498-653. This experiment was conducted under non-reducing conditions only and using Immunoblot Buffer Group 1.
