MA5-38621

antibody from Invitrogen Antibodies

Targeting: RAN ARA24, Gsp1, TC4

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-38621

Provider

Invitrogen Antibodies

Product name

RAN Monoclonal Antibody (8D1A6)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

This antibody has been tested in indirect ELISA.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

8D1A6

Vial size

100 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RAN using RAN Monoclonal Antibody (Product # MA5-38621) in HeLa (1), NIH/3T3 (2), A431 (3), C6 (4), Jurkat (5), HeLa (6), COS7 (7), and Jurkat (8) cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis using RAN Monoclonal Antibody (Product # MA5-38621) against human RAN (AA: 1-216) recombinant protein. (Expected MW is 50.4 kDa)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RAN using RAN Monoclonal Antibody (Product # MA5-38621) in HEK293 (1) and RAN (AA: 1-216)-human IgG-Fc transfected HEK293 (2) cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RAN using RAN Monoclonal Antibody (Product # MA5-38621) in HeLa (1), NIH/3T3 (2), A431 (3), C6 (4), Jurkat (5), HeLa (6), COS7 (7), and Jurkat (8) cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis using RAN Monoclonal Antibody (Product # MA5-38621) against human RAN (AA: 1-216) recombinant protein. (Expected MW is 50.4 kDa)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RAN using RAN Monoclonal Antibody (Product # MA5-38621) in HEK293 (1) and RAN (AA: 1-216)-human IgG-Fc transfected HEK293 (2) cell lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of RAN in GC-7901 cells using RAN Monoclonal Antibody (Product # MA5-38621) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Product # 35503)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of RAN in GC-7901 cells using RAN Monoclonal Antibody (Product # MA5-38621) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Product # 35503)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of RAN in HeLa cells using RAN Monoclonal Antibody (Product # MA5-38621) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Product # 35503)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of RAN in HepG2 cells using RAN Monoclonal Antibody (Product # MA5-38621) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Product # 35503)

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of RAN in paraffin-embedded cervical cancer tissues using RAN Monoclonal Antibody (Product # MA5-38621) and DAB staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of RAN in paraffin-embedded stomach cancer tissues using RAN Monoclonal Antibody (Product # MA5-38621) and DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of RAN in HeLa cells using RAN Monoclonal Antibody (Product # MA5-38621) (green) and negative control (red).