H00001510-M10

antibody from Abnova Corporation

Targeting: CTSE

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001510-M10

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00001510-M10, RRID:AB_1672302

Product name

CTSE monoclonal antibody (M10), clone 2D5

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant CTSE.

Antigen sequence

QGSLHRVPLRRHPTLKKKLRARSQLSEFWKSHNLD
MIQFTESCSMDQSAKEPLINYLDMEYFGTISIGSP
PQNFTVIFDTGSSNLWVPSVYCTSPACKTHSRFQP
SQSSTYSQPGQSFSIQYGTGSLSGIIGADQVSVEG
LTVVGQQFGESVTEPGQTLVDAEFDGILGLGYPSL
AVGGVTPVFDNMMAQNLVDLPMFSVYMSSNPEGGA
GSELIFGGYDHSHFSGSLNWVPVTKQAYWQIALDN
IQVGGTVMFCSEGCQAIVDTGTSLITGPSDKIKQL
QNAIGAAPVDGEYAVECANLNVMPDVTFTINGVPY
TLSPTAYTLLDFVDGMQFCSSGFQGLDIHPPAGPL
WILGDVFIRQFYSVFDRGNNRVGLAPAVP


Isotype

IgG

Antibody clone number

2D5

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of CTSE expression in transfected 293T cell line by CTSE monoclonal antibody (M10), clone 2D5.Lane 1: CTSE transfected lysate (Predicted MW: 42.8 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged CTSE is 1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol