11585-1-AP

antibody from Invitrogen Antibodies

Targeting: SRP68

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

11585-1-AP

Provider

Invitrogen Antibodies

Product name

SRP68 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MSEVEWRGR TVPVKIDKVR IFLLGLADNE AAIVQAESEE TKERLFESML SECRDAIQVV REELKPDQKQ RDYILEGEPG KVSNLQYLHS YLTYIKLSTA IKRNENMAKG LQRALLQQQP EDDSKRSPRP QDLIRLYDII LQNLVELLQL PGLEEDKAFQ KEIGLKTLVF KAYRCFFIAQ SYVLVKKWSE ALVLYDRVLK YANEVNSDAG AFKNSLKDLP DVQELITQVR SEKCSLQAAA ILDANDAHQT ETSSSQVKDN KPLVERFETF CLDPSLVTKQ ANLVHFPPGF QPIPCKPLFF DLALNHVAFP PLEDKLEQKT KSGLTGYIKG IFGFRS (262-596 aa encoded by BC020238)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.4 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 11585-1-AP (SRP68 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 11585-1-AP (SRP68 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA) fixed HepG2 cells using 11585-1-AP (SRP68 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 11585-1-AP (SRP68 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 11585-1-AP ( SRP68 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-SRP68 (IP:11585-1-AP, 4ug; Detection:11585-1-AP 1:400) with mouse kidney tissue lysate 4000ug.