10670-1-AP

antibody from Invitrogen Antibodies

Targeting: SPHK1 SPHK

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

10670-1-AP

Provider

Invitrogen Antibodies

Product name

SPHK1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: HEVVNGLME RPDWETAIQK PLCSLPAGSG NALAASLNHY AGYEQVTNED LLTNCTLLLC RRLLSPMNLL SLHTASGLRL FSVLSLAWGF IADVDLESEK YRRLGEMRFT LGTFLLLAAL RTYRGRLAYL PVGRVGSKTP ASPVVVQQGP VDAHLVPLEE PVPSHWTVVP DEDFVLVLAL LHSHLGSEMF AAPMGRCAAG VMHLFYVRAG VSRAMLLRLF LAMEKGRHME YECPYLVYVP VVAFRLEPKD GKGVFAVDGE LMVSEAVQGQ VHPNYFWMVS GCVEPPPSWK PQQMPPPEEP L (85-384 aa encoded by BC008040)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.17 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

L02 cells were subjected to SDS PAGE followed by western blot with 10670-1-AP ( SPHK1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed L02 cells using 10670-1-AP (SPHK1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed L02 cells using 10670-1-AP (SPHK1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 10670-1-AP (SPHK1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 10670-1-AP (SPHK1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using (Product # 10670-1-AP) (SPHK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-SPHK1 (IP:10670-1-AP, 4ug; Detection:10670-1-AP 1:300) with L02 cells lysate 2560 ug.