NBP1-88637

antibody from Novus Biologicals

Targeting: TCF21 bHLHa23, POD1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

NBP1-88637

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-88637, RRID:AB_11008184

Product name

Rabbit Polyclonal TCF21 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human TCF21 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Characterization of TCF21 Downstream Target Regions Identifies a Transcriptional Network Linking Multiple Independent Coronary Artery Disease Loci.

Sazonova O, Zhao Y, Nürnberg S, Miller C, Pjanic M, Castano VG, Kim JB, Salfati EL, Kundaje AB, Bejerano G, Assimes T, Yang X, Quertermous T
PLoS genetics 2015 May;11(5):e1005202

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Coronary Artery Disease Associated Transcription Factor TCF21 Regulates Smooth Muscle Precursor Cells That Contribute to the Fibrous Cap.

Nurnberg ST, Cheng K, Raiesdana A, Kundu R, Miller CL, Kim JB, Arora K, Carcamo-Oribe I, Xiong Y, Tellakula N, Nanda V, Murthy N, Boisvert WA, Hedin U, Perisic L, Aldi S, Maegdefessel L, Pjanic M, Owens GK, Tallquist MD, Quertermous T
PLoS genetics 2015 May;11(5):e1005155

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TCF21 Antibody [NBP1-88637] - Analysis in control (vector only transfected HEK293T lysate) and tCF21 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TCF21 Antibody [NBP1-88637] - Analysis in control (vector only transfected HEK293T lysate) and TCF21 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: TCF21 Antibody [NBP1-88637] - Staining of human cerebral cortex shows no nuclear positivity in either neurons or glial cells as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: TCF21 Antibody [NBP1-88637] - Staining of human endometrium shows moderate nuclear positivity in a subset of glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: TCF21 Antibody [NBP1-88637] - Staining of human kidney shows moderate nuclear positivity in a subset of tubules as well as in some glomerular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: TCF21 Antibody [NBP1-88637] - Staining of human lung shows strong nuclear positivity in a subset of pneumocytes.