PAB21299

antibody from Abnova Corporation

Targeting: NCKAP1 HEM2, Nap1, NAP125

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB21299

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB21299, RRID:AB_10964653

Product name

NCKAP1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant NCKAP1.

Antigen sequence

LSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPR
ETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQK
SENISPEEEY


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate).Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with NCKAP1 polyclonal antibody (Cat # PAB21299).

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebellum with NCKAP1 polyclonal antibody (Cat # PAB21299) shows strong cytoplasmic positivity in Purkinje cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)