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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-82726 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NCKAP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LSFRSLAQEA LRDVLSYHIP FLVSSIEDFK DHIPRETDMK VAMNVYELSS AAGLPCEIDP ALVVALSSQK SENISPEEEY
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.3 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NCKAP1 by a NCKAP1 polyclonal antibody (Product # PA5-82726). Analysis in control (vector only transfected HEK293T lysate) and nCKAP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415547).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NCKAP1 in U-2 OS cells using a NCKAP1 polyclonal antibody (Product # PA5-82726). The analysis shows localization to cytosol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NCKAP1 in human cerebral cortex and skeletal muscle tissues using a NCKAP1 polyclonal antibody (Product # PA5-82726). Corresponding RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NCKAP1 in human cerebral cortex using a NCKAP1 polyclonal antibody (Product # PA5-82726). The analysis shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NCKAP1 in human skeletal muscle using a NCKAP1 polyclonal antibody (Product # PA5-82726). The analysis shows low expression as expected.
