12140-1-AP

antibody from Invitrogen Antibodies

Targeting: NCKAP1 HEM2, Nap1, NAP125

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

12140-1-AP

Provider

Invitrogen Antibodies

Product name

NCKAP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: HGEPTITSL YTNWYLETLL RQVSNGHIAY FPAMKAFVNL PTENELTFNA EEYSDISEMR SLSELLGPYG MKFLSESLMW HISSQVAELK KLVVENVDVL TQMRTSFDKP DQMAALFKRL SSVDSVLKRM TIIGVILSFR SLAQEALRDV LSYHIPFLVS SIEDFKDHIP RETDMKVAMN VYELSSAAGL PCEIDPALVV ALSSQKSENI SPEEEYKIAC LLMVFVAVSL PTLASNVMSQ YSPAIEGHCN NIHCLAKAIN QIAAALFTIH KGSIEDRLKE FLALASSSLL KIGQETDKTT TRNRESVYLL LDMIVQESPF LTMDLLESCF PYVLLRNAYH AVYKQSVTSS A (779-1129 aa encoded by BC015025)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 12140-1-AP (NCKAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 12140-1-AP (NCKAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 12140-1-AP (NCKAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human heart tissue slide using 12140-1-AP (NAP1;NCKAP1 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 12140-1-AP (NAP1;NCKAP1 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human heart tissue slide using 12140-1-AP ( NAP1;NCKAP1 Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 12140-1-AP ( NAP1;NCKAP1 Antibody) at dilution of 1:50 (under 40x lens).