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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
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- Product number
- PA5-78022 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CYR61 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: HeLa (1X Brefeldin A for 24hr)
- Concentration
- 0.69 mg/mL
Submitted references Cul4A-DDB1-mediated monoubiquitination of phosphoglycerate dehydrogenase promotes colorectal cancer metastasis via increased S-adenosylmethionine.
Zhang Y, Yu H, Zhang J, Gao H, Wang S, Li S, Wei P, Liang J, Yu G, Wang X, Li X, Li D, Yang W
The Journal of clinical investigation 2021 Nov 1;131(21)
The Journal of clinical investigation 2021 Nov 1;131(21)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CYR61 in untreated (-) and treated (+) HeLa whole cell extracts using 30 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with CYR61 polyclonal antibody (Product # PA5-78022) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CYR61 Polyclonal Antibody (Product # PA5-78022). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 Polyclonal Antibody (Product # PA5-78022) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CYR61 Polyclonal Antibody detects CYR61 protein by western blot analysis. Un-treated (-) and treated (+, 1x Brefeldin A treatment for 24 hrs) HeLa whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 Polyclonal Antibody (Product # PA5-78022) diluted by 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti- CYR61 Polyclonal Antibody (Product # PA5-8022) and a ~42 kDa band corresponding to CYR61 was increased upon treating the cells with secretion blockers (FLI and PTI) in MDA-MB-231 when compared to MCF7. Uncharacterized bands (*) were observed at ~ 80, 35 kDa. Whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), MDA-MB-231 treated with FLI (10µM for 4 hr) (Lane 2), MDA-MB-231 treated with PTI (1X for 4 hr) (Lane 3), MCF7 (Lane 4), MCF7 treated with FLI (10µM for 4 hr) (Lane 5) and MCF7 treated with PTI (1X for 4 hr) (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CYR61 in HeLa cells. Samples were treated with 4% paraformaldehyde at RT for 15 min and incubated with CYR61 polyclonal antibody (Product # PA5-78022) using a dilution of 1:200, followed by Hoechst.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CYR61 Polyclonal Antibody detects CYR61 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CYR61 protein stained by CYR61 Polyclonal Antibody (Product # PA5-78022) diluted at 1:200. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CYR61 Polyclonal Antibody detects CYR61 protein at cytoplasm in rat intestine by immunohistochemical analysis. Sample: Paraffin-embedded rat intestine. CYR61 Polyclonal Antibody (Product # PA5-78022) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CYR61 Polyclonal Antibody detects secreted CYR61 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. CYR61 stained by CYR61 Polyclonal Antibody (Product # PA5-78022) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CYR61 Polyclonal Antibody detects secreted CYR61 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. CYR61 stained by CYR61 Polyclonal Antibody (Product # PA5-78022) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
